Thallium(I) treatment induces nucleolar stress to stop protein synthesis and cell growth
Identifieur interne : 000374 ( Main/Exploration ); précédent : 000373; suivant : 000375Thallium(I) treatment induces nucleolar stress to stop protein synthesis and cell growth
Auteurs : Yi-Ting Chou ; Kai-Yin LoSource :
- Scientific Reports [ 2045-2322 ] ; 2019.
Abstract
Thallium is considered as an emergent contaminant owing to its potential use in the superconductor alloys. The monovalent thallium, Tl(I), is highly toxic to the animals as it can affect numerous metabolic processes. Here we observed that Tl(I) decreased protein synthesis and phosphorylated eukaryotic initiation factor 2α. Although Tl(I) has been shown to interact with the sulfhydryl groups of proteins and cause the accumulation of reactive oxygen species, it did not activate endoplasmic reticulum stress. Notably, the level of 60S ribosomal subunit showed significant under-accumulation after the Tl(I) treatment. Given that Tl(I) shares similarities with potassium in terms of the ionic charge and atomic radius, we proposed that Tl(I) occupies certain K+-binding sites and inactivates the ribosomal function. However, we observed neither activation of ribophagy nor acceleration of the proteasomal degradation of 60S subunits. On the contrary, the ribosome synthesis pathway was severely blocked, i.e., the impairment of rRNA processing, deformed nucleoli, and accumulation of 60S subunits in the nucleus were observed. Although p53 remained inactivated, the decreased c-Myc and increased p21 levels indicated the activation of nucleolar stress. Therefore, we proposed that Tl(I) interfered the ribosome synthesis, thus resulting in cell growth inhibition and lethality.
Url:
DOI: 10.1038/s41598-019-43413-1
PubMed: 31061518
PubMed Central: 6502789
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000173
- to stream Pmc, to step Curation: 000173
- to stream Pmc, to step Checkpoint: 000337
- to stream Ncbi, to step Merge: 000599
- to stream Ncbi, to step Curation: 000599
- to stream Ncbi, to step Checkpoint: 000599
- to stream Main, to step Merge: 000374
- to stream Main, to step Curation: 000374
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Thallium(I) treatment induces nucleolar stress to stop protein synthesis and cell growth</title><author><name sortKey="Chou, Yi Ting" sort="Chou, Yi Ting" uniqKey="Chou Y" first="Yi-Ting" last="Chou">Yi-Ting Chou</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Lo, Kai Yin" sort="Lo, Kai Yin" uniqKey="Lo K" first="Kai-Yin" last="Lo">Kai-Yin Lo</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31061518</idno><idno type="pmc">6502789</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502789</idno><idno type="RBID">PMC:6502789</idno><idno type="doi">10.1038/s41598-019-43413-1</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000173</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000173</idno><idno type="wicri:Area/Pmc/Curation">000173</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000173</idno><idno type="wicri:Area/Pmc/Checkpoint">000337</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000337</idno><idno type="wicri:Area/Ncbi/Merge">000599</idno><idno type="wicri:Area/Ncbi/Curation">000599</idno><idno type="wicri:Area/Ncbi/Checkpoint">000599</idno><idno type="wicri:Area/Main/Merge">000374</idno><idno type="wicri:Area/Main/Curation">000374</idno><idno type="wicri:Area/Main/Exploration">000374</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Thallium(I) treatment induces nucleolar stress to stop protein synthesis and cell growth</title><author><name sortKey="Chou, Yi Ting" sort="Chou, Yi Ting" uniqKey="Chou Y" first="Yi-Ting" last="Chou">Yi-Ting Chou</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Lo, Kai Yin" sort="Lo, Kai Yin" uniqKey="Lo K" first="Kai-Yin" last="Lo">Kai-Yin Lo</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Thallium is considered as an emergent contaminant owing to its potential use in the superconductor alloys. The monovalent thallium, Tl(I), is highly toxic to the animals as it can affect numerous metabolic processes. Here we observed that Tl(I) decreased protein synthesis and phosphorylated eukaryotic initiation factor 2α. Although Tl(I) has been shown to interact with the sulfhydryl groups of proteins and cause the accumulation of reactive oxygen species, it did not activate endoplasmic reticulum stress. Notably, the level of 60S ribosomal subunit showed significant under-accumulation after the Tl(I) treatment. Given that Tl(I) shares similarities with potassium in terms of the ionic charge and atomic radius, we proposed that Tl(I) occupies certain K<sup>+</sup>-binding sites and inactivates the ribosomal function. However, we observed neither activation of ribophagy nor acceleration of the proteasomal degradation of 60S subunits. On the contrary, the ribosome synthesis pathway was severely blocked, i.e., the impairment of rRNA processing, deformed nucleoli, and accumulation of 60S subunits in the nucleus were observed. Although p53 remained inactivated, the decreased c-Myc and increased p21 levels indicated the activation of nucleolar stress. Therefore, we proposed that Tl(I) interfered the ribosome synthesis, thus resulting in cell growth inhibition and lethality.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Galvan Arzate, S" uniqKey="Galvan Arzate S">S Galvan-Arzate</name></author><author><name sortKey="Santamaria, A" uniqKey="Santamaria A">A Santamaria</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cvjetko, P" uniqKey="Cvjetko P">P Cvjetko</name></author><author><name sortKey="Cvjetko, I" uniqKey="Cvjetko I">I Cvjetko</name></author><author><name sortKey="Pavlica, M" uniqKey="Pavlica M">M Pavlica</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peter, Al" uniqKey="Peter A">AL Peter</name></author><author><name sortKey="Viraraghavan, T" uniqKey="Viraraghavan T">T Viraraghavan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nriagu, Jo" uniqKey="Nriagu J">JO Nriagu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheam, V" uniqKey="Cheam V">V Cheam</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Zhang, Bg" uniqKey="Zhang B">BG Zhang</name></author><author><name sortKey="Long, Jp" uniqKey="Long J">JP Long</name></author><author><name sortKey="Zhang, Xm" uniqKey="Zhang X">XM Zhang</name></author><author><name sortKey="Chen, Gl" uniqKey="Chen G">GL Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mulkey, Jp" uniqKey="Mulkey J">JP Mulkey</name></author><author><name sortKey="Oehme, Fw" uniqKey="Oehme F">FW Oehme</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pino, Mt" uniqKey="Pino M">MT Pino</name></author><author><name sortKey="Verstraeten, Sv" uniqKey="Verstraeten S">SV Verstraeten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hanzel, Ce" uniqKey="Hanzel C">CE Hanzel</name></author><author><name sortKey="Gubiani, Mfa" uniqKey="Gubiani M">MFA Gubiani</name></author><author><name sortKey="Verstraeten, Sv" uniqKey="Verstraeten S">SV Verstraeten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hanzel, Ce" uniqKey="Hanzel C">CE Hanzel</name></author><author><name sortKey="Verstraeten, Sv" uniqKey="Verstraeten S">SV Verstraeten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eskandari, Mr" uniqKey="Eskandari M">MR Eskandari</name></author><author><name sortKey="Pourahmad, J" uniqKey="Pourahmad J">J Pourahmad</name></author><author><name sortKey="Daraei, B" uniqKey="Daraei B">B Daraei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pourahmad, J" uniqKey="Pourahmad J">J Pourahmad</name></author><author><name sortKey="Eskandari, Mr" uniqKey="Eskandari M">MR Eskandari</name></author><author><name sortKey="Daraei, B" uniqKey="Daraei B">B Daraei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Verstraeten, Sv" uniqKey="Verstraeten S">SV Verstraeten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Klein, Dj" uniqKey="Klein D">DJ Klein</name></author><author><name sortKey="Moore, Pb" uniqKey="Moore P">PB Moore</name></author><author><name sortKey="Steitz, Ta" uniqKey="Steitz T">TA Steitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weiss, Rl" uniqKey="Weiss R">RL Weiss</name></author><author><name sortKey="Kimes, Bw" uniqKey="Kimes B">BW Kimes</name></author><author><name sortKey="Morris, Dr" uniqKey="Morris D">DR Morris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hultin, T" uniqKey="Hultin T">T Hultin</name></author><author><name sortKey="Naslund, Ph" uniqKey="Naslund P">PH Naslund</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hultin, T" uniqKey="Hultin T">T Hultin</name></author><author><name sortKey="Naslund, Ph" uniqKey="Naslund P">PH Naslund</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gerhardy, S" uniqKey="Gerhardy S">S Gerhardy</name></author><author><name sortKey="Menet, Am" uniqKey="Menet A">AM Menet</name></author><author><name sortKey="Pena, C" uniqKey="Pena C">C Pena</name></author><author><name sortKey="Petkowski, Jj" uniqKey="Petkowski J">JJ Petkowski</name></author><author><name sortKey="Panse, Vg" uniqKey="Panse V">VG Panse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ciganda, M" uniqKey="Ciganda M">M Ciganda</name></author><author><name sortKey="Williams, N" uniqKey="Williams N">N Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Strunk, Bs" uniqKey="Strunk B">BS Strunk</name></author><author><name sortKey="Karbstein, K" uniqKey="Karbstein K">K Karbstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woolford, Jl" uniqKey="Woolford J">JL Woolford</name></author><author><name sortKey="Baserga, Sj" uniqKey="Baserga S">SJ Baserga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Planta, Rj" uniqKey="Planta R">RJ Planta</name></author><author><name sortKey="Mager, Wh" uniqKey="Mager W">WH Mager</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sengupta, J" uniqKey="Sengupta J">J Sengupta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ben Shem, A" uniqKey="Ben Shem A">A Ben-Shem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schmidt, Ek" uniqKey="Schmidt E">EK Schmidt</name></author><author><name sortKey="Clavarino, G" uniqKey="Clavarino G">G Clavarino</name></author><author><name sortKey="Ceppi, M" uniqKey="Ceppi M">M Ceppi</name></author><author><name sortKey="Pierre, P" uniqKey="Pierre P">P Pierre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walter, P" uniqKey="Walter P">P Walter</name></author><author><name sortKey="Ron, D" uniqKey="Ron D">D Ron</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tanida, I" uniqKey="Tanida I">I Tanida</name></author><author><name sortKey="Ueno, T" uniqKey="Ueno T">T Ueno</name></author><author><name sortKey="Kominami, E" uniqKey="Kominami E">E Kominami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ossareh Nazari, B" uniqKey="Ossareh Nazari B">B Ossareh-Nazari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lindstrom, Ms" uniqKey="Lindstrom M">MS Lindstrom</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez Corona, U" uniqKey="Rodriguez Corona U">U Rodriguez-Corona</name></author><author><name sortKey="Sobol, M" uniqKey="Sobol M">M Sobol</name></author><author><name sortKey="Rodriguez Zapata, Lc" uniqKey="Rodriguez Zapata L">LC Rodriguez-Zapata</name></author><author><name sortKey="Hozak, P" uniqKey="Hozak P">P Hozak</name></author><author><name sortKey="Castano, E" uniqKey="Castano E">E Castano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nariai, M" uniqKey="Nariai M">M Nariai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Karbstein, K" uniqKey="Karbstein K">K Karbstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="James, A" uniqKey="James A">A James</name></author><author><name sortKey="Wang, Yb" uniqKey="Wang Y">YB Wang</name></author><author><name sortKey="Raje, H" uniqKey="Raje H">H Raje</name></author><author><name sortKey="Rosby, R" uniqKey="Rosby R">R Rosby</name></author><author><name sortKey="Dimario, P" uniqKey="Dimario P">P DiMario</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dai, Ms" uniqKey="Dai M">MS Dai</name></author><author><name sortKey="Arnold, H" uniqKey="Arnold H">H Arnold</name></author><author><name sortKey="Sun, Xx" uniqKey="Sun X">XX Sun</name></author><author><name sortKey="Sears, R" uniqKey="Sears R">R Sears</name></author><author><name sortKey="Lu, H" uniqKey="Lu H">H Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bassler, J" uniqKey="Bassler J">J Bassler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bassler, J" uniqKey="Bassler J">J Bassler</name></author><author><name sortKey="Kallas, M" uniqKey="Kallas M">M Kallas</name></author><author><name sortKey="Hurt, E" uniqKey="Hurt E">E Hurt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Manikas, Rg" uniqKey="Manikas R">RG Manikas</name></author><author><name sortKey="Thomson, E" uniqKey="Thomson E">E Thomson</name></author><author><name sortKey="Thoms, M" uniqKey="Thoms M">M Thoms</name></author><author><name sortKey="Hurt, E" uniqKey="Hurt E">E Hurt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matsuo, Y" uniqKey="Matsuo Y">Y Matsuo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saveanu, C" uniqKey="Saveanu C">C Saveanu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, S" uniqKey="Wu S">S Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hedges, J" uniqKey="Hedges J">J Hedges</name></author><author><name sortKey="West, M" uniqKey="West M">M West</name></author><author><name sortKey="Johnson, Aw" uniqKey="Johnson A">AW Johnson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kallstrom, G" uniqKey="Kallstrom G">G Kallstrom</name></author><author><name sortKey="Hedges, J" uniqKey="Hedges J">J Hedges</name></author><author><name sortKey="Johnson, A" uniqKey="Johnson A">A Johnson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Plaisance, V" uniqKey="Plaisance V">V Plaisance</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boulon, S" uniqKey="Boulon S">S Boulon</name></author><author><name sortKey="Westman, Bj" uniqKey="Westman B">BJ Westman</name></author><author><name sortKey="Hutten, S" uniqKey="Hutten S">S Hutten</name></author><author><name sortKey="Boisvert, Fm" uniqKey="Boisvert F">FM Boisvert</name></author><author><name sortKey="Lamond, Ai" uniqKey="Lamond A">AI Lamond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, K" uniqKey="Yang K">K Yang</name></author><author><name sortKey="Yang, J" uniqKey="Yang J">J Yang</name></author><author><name sortKey="Yi, J" uniqKey="Yi J">J Yi</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Van Riggelen, J" uniqKey="Van Riggelen J">J van Riggelen</name></author><author><name sortKey="Yetil, A" uniqKey="Yetil A">A Yetil</name></author><author><name sortKey="Felsher, Dw" uniqKey="Felsher D">DW Felsher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Russo, A" uniqKey="Russo A">A Russo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tafforeau, L" uniqKey="Tafforeau L">L Tafforeau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bruick, Rk" uniqKey="Bruick R">RK Bruick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smirnov, A" uniqKey="Smirnov A">A Smirnov</name></author><author><name sortKey="Entelis, N" uniqKey="Entelis N">N Entelis</name></author><author><name sortKey="Martin, Rp" uniqKey="Martin R">RP Martin</name></author><author><name sortKey="Tarassov, I" uniqKey="Tarassov I">I Tarassov</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chou, Yi Ting" sort="Chou, Yi Ting" uniqKey="Chou Y" first="Yi-Ting" last="Chou">Yi-Ting Chou</name><name sortKey="Lo, Kai Yin" sort="Lo, Kai Yin" uniqKey="Lo K" first="Kai-Yin" last="Lo">Kai-Yin Lo</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000374 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000374 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:6502789
|texte= Thallium(I) treatment induces nucleolar stress to stop protein synthesis and cell growth
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31061518" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |